GrainGenes Reference Report: TAG-108-1392
Reference
TAG-108-1392
Title
One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat
Journal
Theoretical and Applied Genetics
Year
2004
Volume
108
Pages
1392-1400
Author
Gao LF
[ Show all 10 ]
Abstract
Summary: Four hundred and seventy-eight microsatellite markers derived from expressed sequence tags (EST-SSRs) were screened among three mapping populations (W-7984 x Opata 85, WOpop; Lumai x Hanxuan, LHpop; Wenmai x Shanhongmai, WSpop). The number of polymorphic EST-SSR primer pairs found in WOpop, LHpop and WSpop was 92, 58 and 29 respectively. A total of 101 EST-SSR loci amplified from 88 primer sets were distributed over the 20 chromosomes of the reference maps (no markers were located on chromosome 4B). These 101 mapped EST-SSR markers add to the existing 450 microsatellite loci previously mapped in bread wheat. Seventy-four of the 101 loci showed significant similarities to known genes, including 24 genes involved in metabolism, 4 in cellular structures, 9 in stress resistance, 12 in transcription, 2 in development, 2 transporters and 21 storage proteins. Besides gliadin and glutenin, most of the 53 genes with putative functions were mapped for the first time by EST-SSR markers in bread wheat. Sequence alignment of the mapped wheat EST-SSR loci allowed tentative assignment of functionality to the other members of grasses family. Colinearity combined with homology information offers an attractive approach to comparative genomics
External Databases
Pubmed: 14968301
Keyword
actin-depolymerizing factor
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-108-1392
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||