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GrainGenes Reference Report: TAG-108-343

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Reference	TAG-108-343
Title	Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	343-348
Author	Wardrop J Fuller J Powell W Machray GC
Abstract	Summary: Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated
External Databases	Pubmed: 14513221
Keyword	amplification [ Show all 22 ]

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