GrainGenes Reference Report: TAG-107-299
Reference
TAG-107-299
Title
Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi
Journal
Theoretical and Applied Genetics
Year
2003
Volume
107
Pages
299-305
Author
Xia GM Xiang FN Zhou AF Wang HA Chen HM
Abstract
Summary: Suspension-derived protoplasts of Agropyron elongatum irradiated by ultra-violet light (UV) were fused with the suspension-derived protoplasts of Triticum astivum using PEG. Fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. Their hybrid nature was confirmed by analysis of profiles of isozymes, RAPDs, and 5S rDNA spacer sequences, and via GISH analysis. By the procedure described, the phenotype and chromosome number of wheat could be maintained besides transfer of a few chromosomes and chromosomal fragments from the donor A. elongatum. The results above indicated that highly asymmetric fertile hybrid plants and hybrid progenies of wheat were produced via somatic hybridization
External Databases
Pubmed: 12845444
Keyword
5s
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GrainGenes Reference Report: TAG-107-299
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||