GrainGenes Reference Report: TAG-107-84
Reference
TAG-107-84
Title
Detection of single nucleotide polymorphism (SNP) controlling the waxy character in wheat by using a derived cleaved amplified polymorphic sequence (dCAPS) marker
Journal
Theoretical and Applied Genetics
Year
2003
Volume
107
Pages
84-88
Author
Yanagisawa T Kiribuchi-Otobe C Hirano H Suzuki Y Fujita M
Abstract
Summary: We investigated a single nucleotide polymorphism (SNP) in the Wx-D1 gene, which was found in a mutant waxy wheat, and which expressed the Wx-D1 protein (granule-bound starch synthase I) as shown by immunoblot analysis. We also assayed starch synthase activity of granule-bound proteins. Using 22 doubled-haploid (DH) lines and 172 F5 lines derived from the wild type x the mutant, we detected SNP via a PCR-based (dCAPS) marker. Amplified PCR products from Wx-D1 gene-specific primers, followed by mismatched primers designed for dCAPS analysis, were digested with the appropriate restriction enzyme. The two alleles, and the heterozygote genotype were easily and rapidly discriminated by gel-electrophoresis resolution to reveal SNP. All progeny lines that have the SNP of the mutant allele were waxy. Integrating the results of dCAPS analysis, immunoblot analysis and assays of starch synthase activity of granule-bound proteins indicates that the SNP in the Wx-D1 gene was responsible for its waxy character. This dCAPS marker is therefore useful as a marker to introduce the mutant allele into elite breeding lines
External Databases
Pubmed: 12669198
Keyword
allele
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GrainGenes Reference Report: TAG-107-84
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||