GrainGenes Reference Report: TAG-106-1
Reference
TAG-106-1
Title
Pyramiding transgenes for multiple resistance in rice against bacterial blight, yellow stem borer and sheath blight
Journal
Theoretical and Applied Genetics
Year
2002
Volume
106
Pages
1-8
Author
Datta K Baisakh N Maung Thet K Tu J Datta SK
Abstract
Summary: Here we describe the development of transgene-pyramided stable elite rice lines resistant to disease and insect pests by conventional crossing of two transgenic parental lines transformed independently with different genes. The Xa21 gene (resistance to bacterial blight), the Bt fusion gene (for insect resistance) and the chitinase gene (for tolerance of sheath blight) were combined in a single rice line by reciprocal crossing of two transgenic homozygous IR72 lines. F4 plant lines carrying all the genes of interest stably were identified using molecular methods. The identified lines, when exposed to infection caused by Xanthomonas oryzae pv oryzae, showed resistance to bacterial blight. Neonate larval mortality rates of yellow stem borer (Scirpophaga incertulas) in an insect bioassay of the same identified lines were 100%. The identified line pyramided with different genes to protect against yield loss showed high tolerance of sheath blight disease caused by Rhizoctonia solani
Keyword
bacillus thuringiensis
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-106-1
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||