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GrainGenes Reference Report: TAG-105-937

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Reference	TAG-105-937
Title	Expression and inheritance of the wheat Glu-1DX5 gene in transgenic maize
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	937-945
Author	Sangtong V [ Show all 8 ]
Abstract	Summary: We have produced transgenic maize plants containing a wheat Glu-1Dx5 gene encoding the high-molecular-weight glutenin subunit 1Dx5. Analysis by SDS-PAGE showed that a protein similar in size to the wheat 1Dx5 subunit accumulates in the endosperm of transgenic maize from four independent transformation events. This protein reacts with a monoclonal antibody specific to the wheat 1Dx5 subunit and was not detected in nontransgenic controls or in pollen, anthers, leaves or embryos of plants grown from seeds expressing this protein in endosperm. Genomic Southern-blot analysis is consistent with results from SDS-PAGE and indicates that the transgene integration sites are complex and are different in the four events studied. Using the presence of this protein as a phenotypic marker, we studied the inheritance of this gene through three sexual generations. Reciprocal crosses with nontransgenic plants and self-pollinations were performed, and the resulting kernels were analyzed for the presence of the 1Dx5 subunit. These data, together with PCR analysis for the transgene, suggest that the transgene is inefficiently transmitted through pollen in all four events
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00136/paper/s00122-002-1036-8.pdf
Keyword	bombardment [ Show all 28 ]

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