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GrainGenes Reference Report: TAG-104-1003

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Reference	TAG-104-1003
Title	Genes responding to vernalization in hexaploid wheat
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	1003-1010
Author	Shindo C Sasakuma T
Abstract	Summary: Genotype-specific gene expression in response to vernalization in common wheat was examined by the differential display method. Two near-isogenic lines of Vrn-A1 (Vrn-A1 for the spring type and vrn-A1 for the winter type) were treated by vernalization of developing embryos in detached-ear cultures. This treatment was effective to promote vrn-A1 genotypes to head at a time equivalent to that of Vrn-A1. Differential cDNA fragments were isolated by the RT-PCR method from embryos subjected to vernalization treatments for 2- and 4-weeks at DAP10 and DAP20 stages, respectively. Among 110 differential cDNA fragments isolated, 48 were examined for their chromosomal locations and designated as wec (wheat-embryo cold treatment) genes. Seven wec genes showed genotype-specific expression in response to vernalization. The statistical analysis utilizing two recombinant inbred lines showed that four wec genes were significantly associated with heading factors
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00886/paper/s00122-002-0886-4.pdf
Keyword	cdna [ Show all 31 ]

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