GrainGenes Reference Report: TAG-104-720
Reference
TAG-104-720
Title
PCR detection of Hordeum bulbosum introgressions in an H-vulgare background using a retrotransposon-like sequence
Journal
Theoretical and Applied Genetics
Year
2002
Volume
104
Pages
720-726
Author
Johnston PA Pickering RA
Abstract
Summary: Retrotransposon-like sequences are ideal tools for initial screening assays to distinguish between closely related species because of their ubiquitous presence, high copy number, chromosome coverage and rapid sequence evolution. A retrotransposon-like sequence, pSc119.1, cloned from Secale cereale (rye) has been used to obtain PCR primers that are capable of detecting small introgressions of Hordeum bulbosum (bulbous barley grass) chromatin in a Hordeum vulgare (cultivated barley) background. Combining this PCR-based assay with a crude but effective high-throughput DNA extraction has enabled the rapid identification of plants possessing H. bulbosum introgressions from large numbers of progeny from H. vulgare x H. bulbosum crosses. These plants are then further characterized by more-refined cytological, molecular and pathological techniques to locate and map the introgressed chromatin and to evaluate their disease resistance
External Databases
http://link.springer.de/link/service/journals/00122/papers/2104004/21040720.pdf
Keyword
assay
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-104-720
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||