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GrainGenes Reference Report: TAG-104-680

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Reference	TAG-104-680
Title	Identification of new low-molecular-weight glutenin subunit genes in wheat
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	680-687
Author	Ikeda TM Nagamine T Fukuoka H Yano H
Abstract	Summary: To clarify the composition of low-molecular-weight glutenin subunits (LMW-GSs) in a soft wheat cultivar, we cloned and characterized LMW-GS genes from a cDNA library and genomic DNA in Norin 61. Based on alignment of the conserved N- and C- terminal domains of the deduced amino-acid sequences, these genes are classified into 12 groups. One of these groups (group 5), the corresponding gene of which has not been reported previously, contains two additional hydrophobic amino-acid clusters interrupting the N-terminal repetitive domain. Other groups (groups 11 and 12), which were not identified in other cultivars as a protein product, showed all eight cysteines in the C-terminal conserved domain. With specific primer sets for these groups it was revealed that Glu-D3 and Glu-A3 encoded the former and the latter, respectively. Both groups of genes were expressed in immature seeds. The presence of these groups of LMW-GSs may affect the dough strength of soft wheat
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040680.pdf
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