GrainGenes Reference Report: TAG-103-1028
Reference
TAG-103-1028
Title
Prediction of sample size to maintain genetic variation in doubled-haploid populations following marker selection
Journal
Theoretical and Applied Genetics
Year
2001
Volume
103
Pages
1028-1036
Author
Campbell AW Campbell PJ Griffin WB Burritt DJ Conner AJ
Abstract
Summary: Marker selection (MS) and doubled-haploid (DH) technologies have the potential to reduce the time taken to breed new cereal cultivars. However, a limiting factor is the potential increased genetic drift. The aim of this study was to design and test a genetic model for predicting the sample sizes needed to maintain genetic variation among DH plants following marker selection. The model estimates the amount of the genome that is fixed during the production of DH populations of a given size using a given number of markers. To test the model, doubled-haploids were produced from wheat plants selected for three PCR-based markers. When the genetic variation of the DH population (108 plants), produced from 15 selected F2 plants homozygous at three loci, was compared to the genetic variation of an unselected F3 population (200 plants), five of the six measured quantitative traits were identical and normally distributed. This model should prove to be a valid breeding tool, allowing a breeder to apply MS to a breeding programme and estimate the minimum DH population sizes required for minimal loss of genetic variation through genetic drift
Keyword
assisted selection
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-103-1028
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||