GrainGenes Reference Report: TAG-103-618
Reference
TAG-103-618
Title
AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat
Journal
Theoretical and Applied Genetics
Year
2001
Volume
103
Pages
618-624
Author
Prins R Groenewald JZ Marais GF Snape JW Koebner RMD
Abstract
Summary: Amplified fragment length polymorphism (AFLP) markers were used to enrich the map of the wheat chromosomal region containing the Thinopyrum-derived Lr19 leaf rust resistance gene. The region closest to Lr19 was targeted through the use of deletion and recombinant lines of the translocated segment. One of the AFLP bands thus identified was converted into a sequence-tagged-site (STS) marker. This assay generated a 130-bp PCR fragment in all Lr19-carrying lines tested, except for one deletion mutant, while non-carrier template failed to amplify any product. This sequence represents the first marker to map on the distal side of Lr19 on chromosome 7el1. The conversion process of AFLP fragments to STS markers was technically difficult, mainly because of the presence of contaminating fragments. Various approaches were taken to reduce the frequency of false positives and to identify the correct clone. We were able to formulate a general verification strategy prior to clone sequencing. Various other factors causing problems with converting AFLP bands to an STS assays are also discussed
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1103004/11030618.htm
Locus
Lr19 Sr25
Probe
STSLr19-130
Keyword
aflp
[ Show all 41 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-103-618
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||