GrainGenes Reference Report: TAG-102-320
Reference
TAG-102-320
Title
Assignment of RFLP linkage groups to chromosomes using monosomic F-1 analysis in hexaploid oat
Journal
Theoretical and Applied Genetics
Year
2001
Volume
102
Pages
320-326
Author
Fox SL Jellen EN Kianian SF Rines HW Phillips RL
Abstract
Summary: The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population. Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes. In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally, the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome
External Databases
https://link.springer.com/content/pdf/10.1007/s001220051648.pdf
Germplasm
Kanota-1 (oat) Ogle-C (oat)
Keyword
alleles
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-102-320
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||