GrainGenes Reference Report: TAG-102-177
Reference
TAG-102-177
Title
Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)
Journal
Theoretical and Applied Genetics
Year
2001
Volume
102
Pages
177-186
Author
Nachit MM
[ Show all 14 ]
Abstract
Summary: Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs), and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham 1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total, 306 markers have been placed on the linkage map--138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1% of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding for resistance to biotic and abiotic stresses and for improvement of grain quality
External Databases
https://link.springer.com/content/pdf/10.1007/s001220051633.pdf https://doi.org/10.1007/s001220051633
Gene
Lpx-B1 (Triticum) Lpx-B2 (Triticum)
Locus
Xcnl9(Pdc)-2B Xcn110(Lpx-1)-4B
Map Data
T. turgidum, JK x Cham1
Probe
CNL9
Keyword
5a
[ Show all 67 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-102-177
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||