GrainGenes Reference Report: TAG-100-545
Reference
TAG-100-545
Title
Application of fluorescence based semi-automated AFLP analysis in barley and wheat
Journal
Theoretical and Applied Genetics
Year
2000
Volume
100
Pages
545-551
Author
Schwarz G
[ Show all 7 ]
Abstract
Summary: Genetic mapping and the selection of closely linked molecular markers for important agronomic traits require efficient, large-scale genotyping methods. A semi-automated multifluorophore technique was applied for genotyping AFLP marker loci in barley and wheat. In comparison to conventional 33P-based AFLP analysis the technique showed a higher resolution of amplicons, thus increasing the number of distinguishable fragments. Automated sizing of the same fragment in different lanes or different gels showed high conformity, allowing subsequent unambigous allele-typing. Simultaneous electrophoresis of different AFLP samples in one lane (multi-mixing), as well as simultaneous amplification of AFLP fragments with different primer combinations in one reaction (multiplexing), displayed consistent results with respect to fragment number, polymorphic peaks and correct size-calling. The accuracy of semi-automated co-dominant analysis for hemizygous AFLP markers in an F2 population was too low, proposing the use of dominant allele-typing defaults. Nevertheless, the efficiency of genetic mapping, especially of complex plant genomes, will be accelerated by combining the presented genotyping procedures
External Databases
http://link.springer.de/link/service/journals/00122/bibs/0100003/01000545.htm
Keyword
aflp
[ Show all 40 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-100-545
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||