GrainGenes Reference Report: TAG-100-494
Reference
TAG-100-494
Title
Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance
Journal
Theoretical and Applied Genetics
Year
2000
Volume
100
Pages
494-497
Author
Borner A Chebotar S Korzun V
Abstract
Summary: The genetic identity of eight wheat (Triticum aestivum L.) accessions maintained in the Gatersleben genebank and regenerated up to 24 times was studied by using wheat microsatellite markers (WMS). It was demonstrated that WMS can be used to analyze bulks of seeds stored more than 50 years in a seed reference collection at room temperature. No contamination due to foreign pollen or incorrect handling during the multiplication cycles was discovered. For one accession (TRI 4599) genetic drift was observed, whereas for TRI 249 a heterogenous situation for two markers was maintained over the years. We were able to show that microsatellites can be used as a simple and reliable marker system for the verification of the integrity and genetic stability of genebank accessions
External Databases
http://link.springer.de/link/service/journals/00122/bibs/0100003/01000494.htm
Keyword
accessions
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-100-494
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||