GrainGenes Reference Report: TAG-100-319
Reference
TAG-100-319
Title
Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits
Journal
Theoretical and Applied Genetics
Year
2000
Volume
100
Pages
319-327
Author
Alvarez ML
[ Show all 9 ]
Abstract
Summary: Wheat HMW glutenin subunit genes 1Ax1 and 1Dx5 were introduced, and either expressed or overexpressed, into a commercial wheat cultivar that already expresses five subunits. Six independent transgenic events were obtained and characterized by SDS-PAGE and Southern analysis. The 1Dx5 gene was overexpressed in two events without changes in the other endosperm proteins. Overexpression of 1Dx5 increased the contribution of HMW glutenin subunits to total protein up to 22%. Two events express the 1Ax1 subunit transgene with associated silencing of the 1Ax2* endogenous subunit. In the SDS-PAGE one of them shows a new HMW glutenin band of an apparent Mr lower than that of the 1Dx5 subunit. Southern analysis of the four events confirmed transformation and suggest that the transgenes are present in a low copy number. Silencing of all the HMW glutenin subunits was observed in two different events of transgenic wheat expressing the 1Ax1 subunit transgene and overexpressing the DxS gene. Transgenes and expression patterns were stably transmitted to the progenies in all the events except one where in some of the segregating T2 seeds the silencing of all HMW glutenin subunits was reverted associated with a drastic lost of transgenes from a high to a low copy number. The revertant T2 seeds expressed the five endogenous subunits plus the 1Ax1 transgene
Keyword
biolistics
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-100-319
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||