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GrainGenes Reference Report: TAG-98-977

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Reference	TAG-98-977
Title	Identification of genetic loci affecting amylose content and agronomic traits on chromosome 4A of wheat.
Journal	Theoretical and Applied Genetics
Year	1999
Volume	98
Pages	977-984
Author	Araki E Miura H Sawada S
Abstract	The present study in bread wheat was undertaken, firstly, to identify chromosomes carrying QTLs controlling 1000 grain weight (GW) and, secondly, to develop molecular marker(s) linked with this trait. Using the genotype Rye Selection111 (RS111), we carried out a monosomic analysis that suggested that 8 chromosomes (1A, 1D, 2B, 4B, 5B, 6B, 7A and 7D) carried QTLs controlling GW, with only 3 of these (1A, 2B, 7A) carrying alleles for high GW. To tag the QTLs present on these chromosomes, we crossed the genotype RS111 with high GW (56.83 g) with the genotype Chinese Spring (CS) with low GW (23.74 g) and obtained 100 RILs. These RILs showed normal distribution for GW. The parental genotypes were analysed with as many as 346 STMS primer pairs for detection of polymorphism. Of these, 267 primer pairs gave scorable amplification products, 63 of which detected polymorphism between the parents. Using each of these 63 primer pairs, we carried out bulked segregant analysis on RILs representing two extremes of the distribution. One primer pair (WMC333) showed an association of the marker locus Xwmc333 with grain weight. This was confirmed through selective genotyping, and the co-segregation data on molecular marker locus Xwmc333 and GW were analysed following a single marker linear regression approach. Significant regression suggested linkage between Xwmc333 and a QTL for GW. The results showed that the above QTL accounted for 15.09% of the variation for GW between the parents. The marker has been located on chromosome arm 1AS, and QTL was designated QGw1.ccsu-1A.
External Databases	Komugi: 0047 https://doi.org/10.1007/s001220051437
Germplasm	CS (Kanto107 4A)
QTL	QEet.ocs-4A.1 [ Show all 9 ]
Keyword	amylose qtls wx-b1 yield

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