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GrainGenes Reference Report: TAG-91-941

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Reference	TAG-91-941
Title	The relationship between physical and genetic distances at the Hor1 and Hor2 loci of barley estimated by two-colour fluorescent in situ hybridization
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	941-946
Author	Pedersen C Linde-Laursen I
Abstract	The hordeins are the major class of storage proteins in barley They are encoded by multigene families The B- and C-hordein loci have been mapped physically to the distal end of chromosome 5 (1I) of cultivated barley by fluorescent in situ hybridization Based on measurements of chromosomal distances between the two hordein loci, the relationship between genetic and physical distances has been estimated to be about 1 mega base pairs per centiMorgan This is four times higher than the mean value for the barley genome as a whole and confirms the tendency to increased recombination in distal chromosome regions. The resolving power of two-colour FISH is discussed. It is concluded that the method is suitable for estimating the relationship between genetic and physical distances of regions of about 10 Mbp or larger.
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