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GrainGenes Reference Report: TAG-91-367

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Reference	TAG-91-367
Title	WIS 2-1A: an ancient retrotransposon in the Triticeae tribe
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	367-373
Author	Monte J Flavell R Gustafson J
Abstract	WIS 2-1A, the first retrotransposon found in wheat, has been recently studied and characterized. Southern hybridization experiments utilizing several species from the Triticeae revealed the presence of homologous sequences in all the taxa tested, showing high levels of interspecific variability and almost no intraspecific differentiation. Further experiments using in situ hybridization in several species showed that the retroposon was almost completely dispersed throughout the genomes tested. These results suggested that WIS 2-1A is an ancient element that probably was present in the unknown common ancestor of the Triticeae and that only under rare circumstances does it become active. DNA fragments homologous to the WIS 2-1A reverse transcriptase gene were isolated from most of the Triticeae species using PCR. The fragments obtained were sequenced and analyzed. Even though the sequence alignment was consistent with the phylogenetic studies made in the past, the genus Thinopyrum showed new evidence for a possible horizontal propagation of the retroelement.
Keyword	amino acid sequence [ Show all 9 ]

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