Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-91-320

[Submit comment/correction]

Reference	TAG-91-320
Title	Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum X Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	320-326
Author	D'Hont A [ Show all 7 ]
Abstract	Molecular markers were used to characterise sugarcane intergeneric hybrids between S officinarum and E arundinaceus Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed.
Keyword	aspartate-aminotransferase [ Show all 13 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.