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GrainGenes Reference Report: TAG-90-1101

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Reference	TAG-90-1101
Title	Characterization of a highly repeated DNA component of perennial oats (Helicototrichon, Poaceae) with sequence similarity to a A-genome-specific satellite DNA of rice (Oryza)
Journal	Theoretical and Applied Genetics
Year	1995
Volume	90
Pages	1101-1105
Author	Grebenstein B Grebenstein O Sauer W Hemleben V
Abstract	The taxonomic relationships among perennial oats (Helictotrichon Besser ex Schultes & Schultes Aveninae, Aveneae, Poaceae) have been studied using highly repeated satellite DNA as a molecular marker Highly repetitive sequences were isolated from restriction endonuclease digests of nuclear DNA of Helictotrichon convolutum, and satellite repeats (approximately 365 bp in length) were cloned, sequenced and compared among each other They exhibited an intraspecific sequence variability of 6-9%. This satellite DNA, CON1, is differentially distributed within the genus Helictotrichon. In species of the subgenus Helictotrichon a high copy number is detectable, whereas in representatives of the subgenera Pratavenastrum and Pubavenastrum the number of copies per genome is rather low. Surprisingly, the satellite DNA repeat CON1 shows 74% sequence similarity to an A-genome specific repetitive DNA of Oryza (rice).
Keyword	chemotaxonomy [ Show all 11 ]

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