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GrainGenes Reference Report: TAG-89-964

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Reference	TAG-89-964
Title	Association of a DNA marker with Hessian fly resistance gene H9 in wheat
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	964-968
Author	Dweikat I [ Show all 6 ]
Abstract	The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker co-segregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization
Keyword	biotypes [ Show all 21 ]

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