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GrainGenes Reference Report: TAG-81-397

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Reference	TAG-81-397
Title	A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat.
Journal	Theoretical and Applied Genetics
Year	1991
Volume	81
Pages	397-400
Author	Harcourt RL Gale MD
Type	Article
Language	English
Abstract	An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an "on-off" polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.
Gene	Sr45 (Triticum)
Locus	Xpsr454-3B
Keyword	chromosome 36 [ Show all 10 ]
Agricola Code	IND91033579
Gene Catalogue Number	343
Cited in	McIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.

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