GrainGenes Reference Report: SAS-37-795
Reference
SAS-37-795
Title
Cloning and characterization of a high-affinity nitrate transporter full-cDNA (TaNRT2.1) from wheat roots
Journal
Scientia Agricultura Sinica
Year
2004
Volume
37
Pages
795-800
Author
Yin L Huang Q Huang H Mi G Berry JO
Abstract
A new nitrate transporter gene (TaNRT2.1) that is expressed in wheat roots was identified, and cloned. Based on the sequence of the pTaAF fragment, a full-length cDNA clone was obtained from wheat root RNA using 5'- and 3'-RACE-PCR. Homologous analyses, using TaNRT2 and known HATS sequences of higher plants, indicated that this gene was a member of the NRT2 family in HATS. Southern blotting revealed that TaNRT2.1 is present as a single copy in the wheat genome. Northern analysis demonstrated that accumulation of TaNRT2.1 mRNA was transiently induced by nitrate, showing a rapid increase by 1 h following NO3- treatment, reaching a maximum at 4 h after treatment, and then decreased to the original level by 24 h. TaNRT2.1 mRNA accumulated in roots, but was not present in leaves
Keyword
clone
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: SAS-37-795
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||