Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: RJG-51-241

[Submit comment/correction]

Reference	RJG-51-241
Title	Aging and loss of germination in rye seeds is accompanied by a decreased fragmentation of nuclear DNA at loop domain boundaries
Journal	Russian Journal of Plant Physiology
Year	2004
Volume	51
Pages	241-248
Author	Andreev IO Spiridonova EV Kunakh VA Solov'yan VT
Abstract	To investigate the processes that occur in the embryo cell nuclei in the course of natural and accelerated aging of rye seeds, the nuclear DNA structural organization into chromatin loop domains was studied. The loss of germination was shown to be accompanied by a decreased excision of chromatin loop domains. The study of chromatin accessibility to DNase I [deoxyribonuclease I] did not reveal any considerable changes in chromatin architecture that would explain the decreased DNA fragmentation at matrix attachment regions. A soluble nuclear protein of ca. 31 kD was found to manifest nuclease activity, which declined with the loss of germination. The study of DNA fragmentation in histone-depleted nuclei (nucleoids) disclosed a nuclease activity resistant to 2 M NaCl extraction and sensitive to the specific inhibitors of DNA topoisomerase II [DNA topoisomerase (ATP-hydrolising)]; the latter activity also declined with aging. It was concluded that the changes in DNA fragmentation patterns in aging seeds were primarily caused by a decreased activity of the enzymes accounting for the excision of chromatin loop domains
Keyword	accelerated aging [ Show all 13 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.