GrainGenes Reference Report: PSS-49-41
Reference
PSS-49-41
Title
The use of bulk segregant analysis to identify a RAPD marker linked to the Mla locus of barley
Journal
Plant Breeding and Seed Science
Year
2004
Volume
49
Pages
41-49
Author
Czembor PC Czembor JH
Abstract
Resistance to powdery mildew, Blumeria graminis f.sp. hordei [ Erysiphe graminis f.sp. hordei ], is a major goal of many barley breeding programmes. Resistance conferred by genes located at Mla locus is commonly used by barley breeders for effective control of powdery mildew. The use of molecular markers may facilitate barley breeding for powdery mildew resistance. In this study, bulked segregant analysis (BSA) was used to determine random amplified polymorphic DNAs (RAPDs) markers linked to Mla locus. Thirty-one homozygous (17 resistant and 14 susceptible) F SUB 3 families from a cross between cv. Pallas and single plant line E 1059-1-1 carrying the gene at the Mla locus were used as plant materials. A total of 385 random 10-mer primers were screened to identify polymorphism between the appropriate resistant and susceptible DNA bulks and parents in BSA analysis. Only one PCR marker OPAA3 SUB 400 (primer sequence: 5prime-TTAGCGCCCC-3prime), amplified in polymerase chain reaction (PCR) proved close linkage and was positioned in distance of 10 cM from Mla locus with 5.0 LOD threshold
Keyword
acclimatization
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PSS-49-41
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||