GrainGenes Reference Report: PRT-22-397
Reference
PRT-22-397
Title
Comparison of Agrobacterium-mediated transformation of four barley cultivars using the GFP and GUS reporter genes
Journal
Plant Cell Reports
Year
2004
Volume
22
Pages
397-402
Author
Murray F Brettell R Matthews P Bishop D Jacobsen J
Abstract
Summary: Experiments were conducted to produce transgenic barley plants following infection of immature embryos with Agrobacterium tumefaciens. Transformed callus was obtained using hygromycin resistance as a selectable marker and either green fluorescent protein (GFP) or (beta)-glucuronidase (GUS) as a reporter. Significantly reduced plant transformation frequencies were obtained with the GFP gene compared to GUS. However, GFP proved to be an excellent reporter of early transformation events and was used to compare four barley cultivars for efficiency in two phases of transformation: the generation of stably transformed barley callus and the regeneration of plantlets from transformed callus. Transformed callus was generated at a high frequency (47-76%) in all four cultivars. Regeneration of transformed plantlets was also achieved for all four cultivars although the frequency was much higher for Golden Promise than for the other three genotypes, reiterating that genotype is an important determinant in the regenerative ability of barley. This study has demonstrated for the first time that Agrobacterium-mediated transformation can be used to transform the Australian cultivars Sloop and Chebec
External Databases
Pubmed: 14530864
Keyword
agrobacterium
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PRT-22-397
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||