GrainGenes Reference Report: PRT-20-961
Reference
PRT-20-961
Title
Isolation and characterization of lipid transfer protein (LTP) genes from a wheat-rye translocation line
Journal
Plant Cell Reports
Year
2002
Volume
20
Pages
961-966
Author
Jang CS
[ Show all 8 ]
Abstract
Summary: Two genes (TaLTP1 and TaLTP2) encoding lipid transfer proteins (LTPs) were isolated from a cDNA library constructed from leaf tissue harvested from 4-week-old seedlings of a wheat-rye near-isogenic line (NIL) involving a translocation of rye chromosome 2RL with wheat 2BS. The spatial and temporal patterns of expression of TaLTP1 and TaLTP2 were examined by Northern blot analysis and in situ hybridization. Both TaLTP1 and TaLTP2 contained a 270-bp open reading frame and encoded a putative LTP precursor molecule of 90 amino acids. Expression of the two LTPs was detected in leaves, stems, and crowns of the NILs but not in the roots. The expression levels of TaLTP1 and TaLTP2 remained constant in response to cold and ABA treatments over a period of 24 h but increased 3 days after the initiation of drought stress. An in situ hybridization study indicated that TaLTP1 was expressed in the cells within the vascular bundles of leaves and in the tissue layers between the vascular bundles in the crowns of the control and drought-treated plants. Expression of TaLTP1 in the tissue layers between the vascular bundles was higher in the drought-treated plants than in the control plants. The results suggested that high levels of expression of TaLTPs in the tissue layers between the vascular bundles might play a role in the drought tolerance response of the wheat crown
Keyword
90
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GrainGenes Reference Report: PRT-20-961
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||