GrainGenes Reference Report: PRT-19-118
Reference
PRT-19-118
Title
Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues
Journal
Plant Cell Reports
Year
1999
Volume
19
Pages
118-127
Author
Rasco-Gaunt S Riley A Barcelo P Lazzeri PA
Abstract
Summary: The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to wheat (Triticum aestivum L.) scutellum and inflorescence tissue. The main factors studied were the DNA/gold precipitation process, bombardment parameters and tissue culture variables. Efficiency of DNA (uidA gene) delivery was assessed by scoring transient GUS expression in bombarded tissues. Of the parameters analysed, amount of plasmid DNA, spermidine concentration, presence of Ca(++) ions, calcium chloride concentration, amount of gold particles, gold particle size, acceleration pressure, chamber vacuum pressure, bombardment distance, osmotic conditioning of tissues and type of auxin had a clear influence on transient gene expression. A bombardment procedure suitable for elite wheat varieties was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues
Keyword
auxin
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PRT-19-118
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||