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GrainGenes Reference Report: PRT-14-253

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Reference	PRT-14-253
Title	Efficient regeneration of fertile plants from protoplasts isolated from microspore culture of barley (Hordeum vulgare L.
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	253-256
Author	Salmenkallio-Marttila M Kauppinen V
Abstract	Cultures of isolated microspores of barley (Hordeum vulgare L cv Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts The protoplasts were cultured embedded in agarose The plating efficiency varied from 0.002% to 0.015%. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.
Keyword	anther-culture [ Show all 7 ]

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