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GrainGenes Reference Report: PRT-12-698

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Reference	PRT-12-698
Title	The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays
Journal	Plant Cell Reports
Year	1993
Volume	12
Pages	698-701
Author	Diaz I Royo J Carbonero P
Abstract	Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the beta-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10% No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control) All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site
Keyword	beta-glucuronidase [ Show all 15 ]

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