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GrainGenes Reference Report: PRT-12-666

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Reference	PRT-12-666
Title	Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment
Journal	Plant Cell Reports
Year	1993
Volume	12
Pages	666-670
Author	Gallo-Meagher M Irvine J
Abstract	Effects of tissue type and promoter strength on transient GUS expression in the sugarcane (Saccharum spp hybrids) cultivar NCo 310 were evaluated following microprojectile bombardment of leaf explants GUS expression was histochemically or fluorometrically measured 48 h after delivery of the uidA gene High levels of GUS expression were obtained in leaf segments isolated from young, expanding sugarcane leaves cultured for 1, 3, or 6 d prior to bombardment The promoter derived from the maize ubiquitin 1 gene (Ubi-1) produced significantly more GUS foci and higher GUS activity levels compared to the recombinant Emu, rice actin 1 (Act1), and CaMV 35S promoters Our transient expression system should facilitate efforts to identify promoters and elements which will regulate desired gene expression patterns in sugarcane and aid in development of an efficient stable transformation system
Keyword	beta-glucuronidase [ Show all 9 ]

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