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GrainGenes Reference Report: PRT-12-366

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Reference	PRT-12-366
Title	Viability, cell division and microcallus formation of barley microspores in culture
Journal	Plant Cell Reports
Year	1993
Volume	12
Pages	366-369
Author	Kao K
Abstract	Barley microspores were viable when cultured in a sugarless medium Adding 2g of glucose to 1l of this medium resulted in a significant reduction in the frequency of viable microspores The frequency of viable microspores was further reduced when 50g of cellobiose, glucose, maltose, melezitose, raffinose or sucrose were added to 1l of the culture medium containing 2g/l glucose Adding 50g of melibiose, Ficoll, polyethylene glycol (PEG) or a combination 50g each of Ficoll and PEG to 1l of the medium containing 2g/l glucose had very little effect on the viability of the microspores Up to 66% of the viable microspores were able to divide and many of these developed into microcalli in the basal medium complemented with melibiose, maltose, melezitose, raffinose, Ficoll, PEG or a combination of Ficoll with PEG Sucrose, cellobiose and glucose added in large quantities inhibited cell division in microspores or destabilized the microspores and only very few of them developed into microcalli The microcalli in the PEG, Ficoll, Ficoll-PEG and melibiose media were smaller in size than those grown in the melezitose, maltose and raffinose media Sustained cell division and microcallus formation were observed in a medium with melibiose or maltose as sole source of sugars.
Keyword	anther-culture [ Show all 17 ]

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