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GrainGenes Reference Report: PPS-135-1753

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Reference	PPS-135-1753
Title	Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses
Journal	Plant Physiology
Year	2004
Volume	135
Pages	1753-1764
Author	Castleden CK [ Show all 9 ]
Abstract	Sucrose-phosphate synthase (SPS) is a key regulatory enzyme in the pathway of sucrose biosynthesis, and has been linked to quantitative trait loci controlling plant growth and yield. In dicotyledonous plants, there are 3 SPS gene families: A, B and C. We report the finding of 5 families of SPS genes in wheat (Triticum aestivum cultivars Hartog and Cadenza) and other monocotyledonous plants from the family Poaceae (Aegilops tauschii, T. turgidum var. durum (T. durum) cv. Fransawi, barley, maize and rice). Three of these form separate subfamilies within the previously described A, B, and C gene families, but the other 2 form a novel and distinctive D family, which on present evidence is only found in the Poaceae. The D-type SPS proteins lack the phosphorylation sites associated with 14-3-3 protein binding and osmotic stress activation, and the linker region between the N-terminal catalytic glucosyltransferase domain and the C-terminal Suc-phosphatase-like domain is 80 to 90 amino acid residues shorter than in the A, B or C types. The D family appears to have arisen after the divergence of mono- and dicotyledonous plants, with a later duplication event resulting in the 2 D-type subfamilies. Each of the SPS gene families in wheat showed different, but overlapping spatial and temporal expression patterns, and in most organs at least 2 different SPS genes are expressed. The analysis of expressed sequence tags indicated similar expression patterns to wheat for each SPS gene family in barley but not in more distantly related grasses. We identified an expressed sequence tag from rice that appears to be derived from an endogenous antisense SPS gene, and this might account for the apparently low level of expression of the related OsSPS11 sense gene, adding to the already extensive list of mechanisms for regulating the activity of SPS in plants
External Databases	Pubmed: 15247374
Keyword	activation [ Show all 34 ]

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