Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PPS-117-679

[Submit comment/correction]

Reference	PPS-117-679
Title	Allele-Dependent Barley Grain Beta-Amylase Activity
Journal	Plant Physiology
Volume	117
Pages	679-685
Author	Erkkila MJ Leah R Ahokas H Cameron-Mills V
Abstract	The wild ancestor of cultivated barley, Hordeum vulgaresubsp. spontaneum (K. Koch) A. & Gr. (H. spontaneum), is a source of wide genetic diversity, including traits that are important for malting quality. A high Beta-amylase trait was previously identified in H. spontaneum strains from Israel, and transferred into the backcross progeny of a cross with the domesticated barley cv Adorra. We have used Southern-blot analysis and Beta-amy1 gene characterization to demonstrate that the high Beta-amylase trait in the backcross line is co-inherited with the Beta-amy1 gene from the H. spontaneumparent. We have analyzed the Beta-amy1 gene organization in various domesticated and wild-type barley strains and identified three distinct Beta-amy1 alleles. Two of these Beta-amy1 alleles were present in modern barley, one of which was specifically found in good malting barley cultivars. The third allele, linked with high grain Beta-amylase activity, was found only in a H. spontaneum strain from the Judean foothills in Israel. The sequences of three isolated Beta-amy1 alleles are compared. The involvement of specific intron III sequences, in particular a 126-bp palindromic insertion, in the allele-dependent expression of Beta-amylase activity in barley grain is proposed.
External Databases	https://doi.org/10.1104/pp.117.2.679
Gene	Bmy1 (Hordeum)
Sequence	AF061203

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.