GrainGenes Reference Report: PPB-47-335
Reference
PPB-47-335
Title
Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants.
Journal
Plant Physiology and Biochemistry
Year
2009
Volume
47
Pages
335-342
Author
Caputo C Criado MV Roberts IN Gelso MA Barneix AJ
Abstract
The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants.
External Databases
http://dx.doi.org/10.1016/j.plaphy.2009.01.003
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PPB-47-335
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||