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GrainGenes Reference Report: PNA-93-2408

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Reference	PNA-93-2408
Title	GSP-1 genes are linked to the grain hardness locus (Ha) on wheat chromosome 5D
Journal	Proceedings of the National Academy of Sciences, USA
Year	1996
Volume	93
Pages	2408-2413
Author	Jolly C Glenn G Rahman S
Abstract	An important determinant of wheat grain quality is the hardness of the grain The trait is controlled by a major locus, Ha, on the short arm of chromosome 5D Purified starch granules from soft-grained wheats have associated with them 15-kDa polypeptides called grain softness proteins (GSPs) or 'friabilins'. Genes that encode one family of closely related GSP polypeptides--GSP-I genes--were mapped using chromosome substitution lines to the group 5 chromosomes An F2 population segregating for hard and soft alleles at the Ha locus on a near-isogenic background was used in a single-seed study of the inheritance of grain softness and of GSP-1 alleles Grain softness versus grain hardness was inherited in a 3:1 ratio. The presence versus absence of GSPs in single seed starch preparations was coinherited with grain softness versus hardness. This showed that grain softness is primarily determined by seed, and not by maternal, genotype. In addition, no recombination was detected in 44 F2 plants between GSP-1 restriction fragment length polymorphisms and Ha alleles. Differences between hard and soft wheat grains in membrane structure and lipid extractability have been described and, of the three characterized proteins that are part of the mixture of 15-kDa polypeptides called GSPs, at least two, and probably all three, are proteins that bind polar lipids. The data are interpreted to suggest that the Ha locus may encode one or more members of a large family of lipid-binding proteins.
External Databases	GSP-1
Allele	Gsp-D1a (Triticum)
Gene	Gsp-A1 (Triticum) Gsp-B1 (Triticum) Gsp-D1 (Triticum)
Keyword	binding protein [ Show all 24 ]

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