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GrainGenes Reference Report: PMP-44-389

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Reference	PMP-44-389
Title	Incompatibility conditioned by the Mla gene in powdery mildew of barley: timing of the effect of cordycepin on hypersensitive cell death
Journal	Physiology and Molecular Plant Pathology
Year	1994
Volume	44
Pages	389-402
Author	Bushnell W Liu Z (2)
Abstract	The effect of cordycepin, an inhibitor of mRNA synthesis, on the hypersensitive host cell death response (HR), was tested with epidermal tissues from barley coleoptiles containing the Mla gene for resistance, inoculated with incompatible race 3 of Erysiphe graminis f.sp hordei In untreated control tissues, HR occurred 18-30 h after inoculation in 61-91% of cells with haustoria The percentage of HR was strongly reduced by cordycepin at 10-31 micromolar but only if applied 4 h after inoculation or before; not if applied 8 h or later Effective cordycepin treatments had no inhibitory effect on the development of germ tubes or appressoria and usually increased the development of haustoria and hyphae. The results indicate that mRNAs required for the HR are synthesized in the host within the first 0-8 h after inoculation and that mRNA synthesis is not required thereafter. The timing of protein synthesis required for the HR in the host could not be determined because the inhibitors of protein synthesis used, blasticidin S and cycloheximide, inhibited parasite development at concentrations required to inhibit the HR.
Keyword	coleoptile [ Show all 10 ]

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