GrainGenes Reference Report: BBR-375-230
Reference
BBR-375-230
Title
Antimicrobial activity of gamma-thionin-like soybean SE60 in E. coli and tobacco plants.
Journal
Biochemical and Biophysical Research Communications
Year
2008
Volume
375
Pages
230-234
Author
Choi Y Choi YD Lee JS
Abstract
The SE60, a low molecular weight, sulfur-rich protein in soybean, is known to be homologous to wheat gamma-purothionin. To elucidate the functional role of SE60, we expressed SE60 cDNA in Escherichia coli and in tobacco plants. A single protein band was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) after anti-FLAG affinity purification of the protein from transformed E. coli. While the control E. coli cells harboring pFLAG-1 showed standard growth with Isopropyl beta-d-1-thiogalactopyranoside (IPTG) induction, E. coli cells expressing the SE60 fusion protein did not grow at all, suggesting that SE60 has toxic effects on E. coli growth. Genomic integration and the expression of transgene in the transgenic tobacco plants were confirmed by Southern and Northern blot analysis, respectively. The transgenic plants demonstrated enhanced resistance against the pathogen Pseudomonas syringae. Taken together, these results strongly suggest that SE60 has antimicrobial activity and play a role in the defense mechanism in soybean plants.
External Databases
http://dx.doi.org/10.1016/j.bbrc.2008.08.001
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: BBR-375-230
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||