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GrainGenes Reference Report: PMB-23-613

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Reference	PMB-23-613
Title	Effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts
Journal	Plant Molecular Biology
Year	1993
Volume	23
Pages	613-618
Author	Rathus C Bower R Birch R
Abstract	Various chimaeric promoter regions coupled to the uidA4 beta-glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts Multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial Emu promoter relative to the CaMV 35S promoter The relative expression strengths of promoters varied substantially between the species. Sugar-cane also differed in some respects from previously tested species in the family Poaceae. For example, in sugar-cane the nopaline synthase and CaMV 35S promoters were of equivalent strength, and insertion of Adh1 intron 1 into the 5' transcribed region decreased expression strength.
Keyword	35s-promoter [ Show all 18 ]

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