GrainGenes Reference Report: PMB-18-585
Reference
PMB-18-585
Title
Structure and expression of the barley lipid transfer protein gene LTP1
Journal
Plant Molecular Biology
Year
1992
Volume
18
Pages
585-589
Author
Skriver K Leah R Muller-Uri F Olsen FL Mundy J
Abstract
We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5' upstream region of Ltp1 is sufficient to direct aleurone- specific expression during late seed development and early germination.
Probe
A1f
Keyword
Aleurone-specific expression
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PMB-18-585
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||