Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PMB-17-1217

[Submit comment/correction]

Reference	PMB-17-1217
Title	Amber codon suppression the in-vivo and in-vitro analysis of two c-hordein genes from barley
Journal	Plant Molecular Biology
Year	1991
Volume	17
Pages	1217-1232
Author	Entwistle J Knudsen S Muller M Cameron-Mills V
Type	Article
Language	English
Abstract	A 1420 bp genomic fragment (.lambda.-horl-17) encompassing a Hor-1 gene encoding a C-hordein polypeptide is presented. The deduced amino acid sequence is 261 residues long. It comprises a 20 amino acid signal peptide, unique NH2- and COOH-terminal regions and a coding region comprised of pentapeptide (PQQPY) and octapeptide (PQQPFPQQ) repeat motifs. The 431 bp of 5' non-coding region contains a 'TATA box' at -105, a 'CACA box' (-181 to -201) and a - 300 prolamin element. In the 3' noncoding region there are two putative polyadenylation signals located 88 and 142 bp downstream of the stop codon. The structure of .lambda.-horl-17 is compared with that of another gene (.lambda.-horl-14) encoding a C-hordein polypeptide, which contains an amber codon interrupting the ORF. A functional assay in which the 5' non-coding regions of the two genes were fused to the .beta.-glucuronidase (GUS) gene demonstrated that both genes were transcriptionally active and that circa 430 bp of the C-hordein promoters were sufficient to drive the expression of the GUS gene in developing barley endosperms. It also demonstrated that both promoters had transcriptional efficiencies comparable with that of the 35S CaMV promoter. The in vitro translation of the coding region of .lambda.-horl-14 in the wheat germ system showed that the premature stop codon could be partially suppressed. The suppression was also demonstrated in a transient expression assay in vivo using isolated barley endosperms.
Probe	lambda-hor1-14 lambda-hor1-17
Keyword	Amino acid sequence [ Show all 10 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.