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GrainGenes Reference Report: PMB-12-285

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Reference	PMB-12-285
Title	Barley aleurone cell development molecular cloning of aleurone-specific complementary DNA from immature grains
Journal	Plant Molecular Biology
Year	1989
Volume	12
Pages	285-294
Author	Jakobsen K [ Show all 6 ]
Type	Article
Language	English
Abstract	The cloning of 11 different homology groups of cDNAs representing genes expressed in aleurone, but not in starchy endosperm of 20-day- old barley grains is described. Among the cDNAs, four are aleurone- specific, while the remaining are also expressed in the embryo, but not in any other part of the plant. Sequence analysis of one of the aleurone-specific clones, B11E, reveals an open reading frame coding for an unidentified 10.4 kDa protein with a putative signal sequence and a possible metal-binding finger. The B11E gene has a high GC content in the 5' leader sequence (63%), as well as in the coding region (70%) compared to known cDNAs from the barley starchy endosperm. Northern analysis of B11E indicates maximum mRNA abundnace around mid-phase of grain development. When isolated immature aleurone/pericarp is incubated in tissue culture medium (MS) the B11E message disappears, indicating a requirement for a diffusible factor from the intact grain for its continued presence.
Probe	B11E

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