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GrainGenes Reference Report: PMB-11-495

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Reference	PMB-11-495
Title	Cloning and characterization of a complementary DNA encoding a messenger rna rapidly-induced by aba in barley aleurone layers
Journal	Plant Molecular Biology
Year	1988
Volume	11
Pages	495-506
Author	Hong B Uknes SJ Ho THD
Type	Article
Language	English
Abstract	Abscisic acid (ABA) inhibits the gibberellic acid induced synthesis of .alpha.-amylase in barley aleurone layers, yet ABA itself induces more than a dozen polypeptides (Lin & Ho, Plant Physiol 82: 289-297, 1986). As part of our effort to elucidate the molecular action of ABA in barley aleurone layers, we have isolated and characterized an ABA-induced cDNA clone, pHV A1. This cDNA clone hybridizes to an RNA species of approximately 1.1 kb from ABA-treated barley aleurone layers. The level of this mRNA is tripled within 40 minutes after ABA treatment, reaches a peak at 8-12 h, and is present up to 48 h. The induction of this mRNA responds to concentrations of ABA as low as 10-9 M, but higher ABA concentrations induce higher expression of this mRNA. The products of hybrid-select translation and in vitro transcription/translation with pHV A1 comigrate on SDS gel as a 27 kDa polypeptide. However, the sequence of pHV A1 indicates that it has an open reading frame encoding a 22 kDa protein. This size discrepancy is probably due to the high content of the basic amino acid, lysine. This notion has been confirmed by two-dimensional gel electrophoresis showing that this polypeptide is one of the most basic proteins in ABA-treated barley aleurone layers. The deduced amino acid sequence of pHV A1 contains nine imperfect repeats 11 amino acids long which share homology with cotton Lea 7 protein (Baker, Steele & Dure, Plant Mol biol, in press). The identity and function of the encoded product of pHV A1 is under investigation.
Probe	pHV A1
Keyword	Abscisic acid [ Show all 8 ]

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