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GrainGenes Reference Report: PLC-58-149

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Reference	PLC-58-149
Title	Regeneration of fertile transgenic tall fescue plants with a stable highly expressed foreign gene
Journal	Plant Cell
Year	2000
Volume	58
Pages	149-154
Author	Kuai B Dalton SJ Bettany AJE Morris P
Abstract	Summary: One hundred and seventeen green tall fescue plants and 37 albino plants were regenerated from a glufosinate ammonium resistant callus clone co-transformed with the bar gene and the gus gene, both driven by the rice actin 1 promoter. The gus gene was not detectable in regenerated plants but the presence of the bar gene in these plants was detected by the polymerase chain reaction and integration of the bar gene into the genome by Southern blot hybridization. A high and stable expression of the bar gene was evident from the assay for phosphinothricin-N-acetyltransferase activity and from spraying plants with glufosinate ammonium herbicide. There was no detectable variation with respect to the level of bar gene expression among these plants. However, no inheritance of the bar gene was found in two populations of outcrossed progenies
Keyword	ammonia [ Show all 20 ]

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