GrainGenes Reference Report: PLA-210-188
Reference
PLA-210-188
Title
Characterization of two cDNA clones encoding isoenzymes of the F1F0-ATPase inhibitor protein of rice mitochondria
Journal
Planta
Year
2000
Volume
210
Pages
188-194
Author
Nakazono M Imamura T Tsutsumi N Sasaki T Hirai A
Abstract
Summary: Two cDNA clones encoding F1F0-ATPase inhibitor proteins, which are loosely associated with the F1 part of the mitochondrial F1F0-ATPase, were characterized from rice (Oryza sativa L. cv. Nipponbare). A Northern hybridization showed that the two genes (designated as IF(1)-1 and IF(1)-2) are transcribed in all the organs examined. However, the steady-state mRNA levels varied among organs. A comparison of the deduced amino acid sequences of the two IF(1) genes and the amino acid sequence of the mature IF1 protein from potato revealed that IF(1)-1 and IF(1)-2 have N-terminal extensions with features that are characteristic of a mitochondrial targeting signal. To determine the subcellular localization of the gene products, the IF(1)-1 or IF(1)-2 proteins were fused in frame to the green fluorescent protein (GFP) or the fused GFP-beta-glucuronidase, and expressed transiently in onion or dayflower epidermal cells. Localized fluorescence was detected in mitochondria, confirming that the two IF(1) proteins are targeted to mitochondria
Keyword
adenosinetriphosphatase
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GrainGenes Reference Report: PLA-210-188
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||