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GrainGenes Reference Report: PLA-192-84

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Reference	PLA-192-84
Title	Transient expression of visible marker genes in meristem cells of wheat embryos after ballistic micro-targeting
Journal	Planta
Year	1994
Volume	192
Pages	84-91
Author	Iglesias V [ Show all 6 ]
Abstract	Seven days after anthesis, the shoot apical meristem of immature embryos of wheat (Triticum aestivum L. is not yet covered by the coleoptile or leaf primordia and provides an optimal target for ballistic micro-targeting Gold particles 12 micromole in diameter at a concentration of 5.10(5) particles per microliter and propelled by 1.10-bar nitrogen penetrated up to four cell layers into embryo apical meristems but produced no deleterious effects on germination The use of diaphragms with internal diameters of 100 or 200 micrometer restricted bombardment to meristem cells or also included surrounding tissues, respectively. The results of transient-expression experiments indicated successful delivery of foreign DNA into meristem cells. Cells of the central zone of the meristem or pro-meristem transiently expressed foreign genes driven by the Cauliflower mosaic virus (CaMV) 35S and rice actin1-D constitutive promoters. Partial plasmolysis before bombardment and slow recovery of normal turgor pressure increased transient-expression frequencies. Meristem cells transiently expressed foreign genes at frequencies 10-fold less than surrounding tissues under identical conditions. Transgenic sectors were observed in both coleoptiles and leaf primordia.
Keyword	developmental-stages [ Show all 12 ]

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