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GrainGenes Reference Report: PLA-189-249

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Reference	PLA-189-249
Title	Half-lives of oat mRNAs in vivo and in a polysome-based in-vitro system
Journal	Planta
Year	1993
Volume	189
Pages	249-256
Author	Byrne D Seeley K Colbert J
Abstract	We have used a cell-free polysome-based in-vitro mRNA-degradation system to investigate the half-lives of plant cell mRNAs In order to establish the fidelity of the in-vitro system, we used cordycepin to determine the in-vivo half-lives of beta-tubulin and actin mRNAs in the primary leaves of 4-d-old etiolated oat (Avena sativa L.) seedlings The in-vitro rank order of half-lives for phytochrome A (45 min), beta-tubulin (105 min), and actin (220 min) mRNAs mimicked the in-vivo rank order A pulse of red light given to excised etiolated primary leaves caused an in-vivo reduction in the half-life of beta-tubulin mRNA The selectivity of the polysome-based system was further demonstrated by the decrease in the half-life of beta-tubulin mRNA (from 105 min to 60 min) induced by a pulse of red light given to the etiolated oat seedlings prior to isolation of polysomes Red light did not affect the apparent half-lives of phytochrome A or actin mRNAs.
Keyword	actin [ Show all 12 ]

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