GrainGenes Reference Report: PLA-189-39
Reference
PLA-189-39
Title
Chalcone synthase and flavonoid products in primary-leaf tissues of rye and maize
Journal
Planta
Year
1993
Volume
189
Pages
39-46
Author
Jahne A Fritzen C Weissenbock G
Abstract
During cell and tissue differentiation of developing rye (Secale cereale L. and maize (Zea mays L. primary leaves, various flavonoids are synthesized and accumulate in both epidermal and mesophyll tissues In order to prove either the biosynthetic autonomy of each tissue type and- or intercellular transport of flavonoids, the tissue distributions of chalcone synthase (CHS:EC 2.3.1.74), the key enzyme of the pathway, and of flavonoids have been comparatively investigated. Monoclonal antibodies raised against CHS from rye were used to relate enzyme activity in a particular tissue extract to the corresponding amount of CHS protein. A close correlation was found between CHS activities and amounts of CHS protein during leaf development and in the various tissues. The simultaneous occurrence of CHS in both epidermal layers as well as in the mesophyll correlated with the accumulation of flavonoid products in these tissues, indicating tissue autonomy of flavonoid biosynthesis. These data are in contrast to previous reports (Knogge and Weissenbock, 1986, Planta 167, 196-205) on primary leaves of oat (Arena sativa) where CHS and several subsequent enzymes were located mainly in the mesophyll whereas major flavonoid products accumulated predominantly in both epidermal cell layers, indicating that intertissue transport of flavonoids might occur.
Keyword
enzyme activity
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GrainGenes Reference Report: PLA-189-39
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||