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GrainGenes Probe Report: H33_parsnp10

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	H33_parsnp10
Reference	Subramanyam S et al. (2016) Targeted Discovery of Single-Nucleotide Polymorphisms in an Unmarked Wheat Chromosomal Region Containing the Hessian Fly Resistance Gene H33 Crop Science 56:1106-1114.
General Remarks	Amplicon size of flanking sequence = 318
Type	KASP
PCR primers	SNP flanking forward CGACCGACTCCACCACCCAT SNP Flanking reverse TGGGCACGAGGAACAAGCG
PCR primers	KASP forward resistant GCAATGAGCAGTGCCAGTGCC KASP forward susceptible GCAATGAGCAGTGCCAGTGCA KASP common reverse CGCCGCGACCCAGCGAGAA
Amplification Conditions	Anneal flanking primers at 60C
Source Gene	H33 (Triticum)